Applicants will understand the principle of microarrays technology and perform them using either Affymetrix or Agilent microarrays platforms and analyze the data in order to identify meaningful information/ biomarkers for a particular pathophysiological context.

• Microarray technology and applications
• The latest generation of cytogenetic microarrays
• Measurement of quantity & quality of RNA, part I
• Overview of the gene expression assay workflow on the Thermo Fisher Scientific Gene Chip System, part II (cRNA purification)
• Overview of the gene expression assay workflow on the Gene Chip System, part III (cDNA purification)
• Overview of the gene expression assay workflow on the Gene Chip System, part IV (hybridization, wash & scan)
• Chromosomal microarray approach to abnormal reads
• Measurement of quantity & quality of DNA , part I
• Overview of the array CGH workflow using Agilent platform, part II (fragmentation & labeling)
• Overview of the array CGH workflow using Agilent platform, part III (purification & hybridization)
• Overview of the array CGH workflow using Agilent platform, part IV (wash, scan & data analysis)
• Bioinformatics analysis

Price: 1500 SR
CME: 13 Hours

Speakers: Dr. Hans Schulten, Dr. Heba Alkhatabi, Dr. Sajjad Karim